Preliminary investigations of the soluble and insoluble organic materials in 3 (carbonaceous) Itokawa particles were conducted and the results indicated that the organic components were largely derived from terrestrial contaminants accrued from sample handling and/or other laboratory procedures. Therefore, a fundamental understanding of the nature of the organic content in Itokawa is still lacking. Itokawa is likely to be the parent body of LL4-6 ordinary chondrites, which indicates that the asteroid has experienced extensive thermal alteration processing. Given this temperature profile, Itokawa offers an excellent study ground to evaluate the correlation between the heating history of asteroid and the syntheses of amino acids - the important biomolecules that are indispensable to life on Earth. In this proposal, we aim to establish a better understanding of the organic and chemical contents of Itokawa through carrying out a range of coordinated analyses involving carefully prioritized analytical techniques including two-step laser desorption/laser ionization mass spectrometry (ultra-L2MS), electron beam microscopy (FE-SEM, EBSD), micro-Raman spectrometry, and chromatography.